Overview - SHENTEK^® Lentivirus Titer p24 ELISA Kit (One-step ELISA)

The Lentivirus Titer p24 ELISA Kit (One-step ELISA) is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a double-antibody sandwich technique to detect Human Immunodeficiency Virus type 1 (HIV-1) p24/Capsid Protein p24. The 96-well strip plate is precoated with a monoclonal antibody specific to HIV-1 p24 / Capsid Protein p24. The Lysis solution, Calibration Standard (or test sample) and the HRP (Horseradish Peroxidase) labeled anti-p24 antibody were simultaneously added to the microtiter plate. Then TMB (3,3',5,5'-tetramethylbenzidine) substrate was added into the reaction, HRP catalyzed the oxidation of TMB by H₂O₂ to produce a blue product. Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the p24 concentration in the Calibration standard and the sample. The concentration of p24 in the sample can be calculated using a dose-response curve.

Introduction - SHENTEK^® Lentivirus Titer p24 ELISA Kit (One-step ELISA)

Specification -SHENTEK^® Lentivirus Titer p24 ELISA Kit (One-step ELISA)