Overview - SHENTEK^® Bovine Serum Albumin (BSA) ELISA Kit

This SHENTEK^® Bovine Serum Albumin (BSA) ELISA Kit is based on the solid-phase enzyme-linked immunosorbent assay (ELISA) with a double-antibody sandwich technique to detect residual bovine serum albumin (BSA). A sheep polyclonal antibody specific to BSA was employed in the assay to capture any remaining BSA impurities in the sample. Both the Calibration standards and test samples were simultaneously added to the microtiter plate coated with the affinity purified capture antibody, and followed by incubation and washing. The biotinylated antibody was added to the microtiter plate to bind the BSA and then reacted with streptavidin labeled HRP (Horseradish Peroxidase). TMB (3,3',5,5' -tetramethylbenzidine) substrate was added into reaction, HRP catalyzed the oxidation of TMB by H₂O₂ to produce a blue product (maximum absorption peak at 655 nm). Then the stop solution was added to terminate the enzymatic reaction, resulting in a yellow colored product (maximum absorption peak at 450 nm). The absorbance values at 450 nm wavelength were positively correlated with the BSA concentration in the calibration standard and the samples. The concentration of BSA in the samples can be calculated using a dose-response curve.

Introduction - SHENTEK^® Bovine Serum Albumin (BSA) ELISA Kit